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GO2 Max | Peak Endurance Support
GO2 Max Is BACK! WADA Compliant & 100% NATURAL What G02 MAX Can Do For You! Increased Energy* Increased Endurance* Recover Faster Between Sets* Last Longer and Train Harder during workouts* The Result... Burn more Fat and Calories / Get in the Shape of Your Life!* Supplement Facts Ingredients NMN works right away to increase performance and doesn't need to be loaded like creatine or beta alanine. NMN is converted into NAD+ which is an important molecule that promotes the process of glycolysis and the kreb's cycle, this means it increases the rate of energy production from our body's reserve of fats and glucose. This well designed study demonstrates that NMN can boost aerobic capacity performance metrics by up to 30% in trained athletes, this beats beta alanine by a long shot. NMN is also known for its anti-aging benefits. NAD+ levels decline rapidly with age, NAD+ levels can be reduced by up to 50% past the age of 30. NMN supplementation potently boosts NAD+ production and can increase insulin sensitivity, reverse mitochondrial dysfunction, and extend the lifespan in aging populations. Nicotinamide Mononucleotide(NMN) NMN is a naturally occurring nucleotide with great potential, it has been shown to improve aerobic exercise capacity, VO2 (rate of oxygen consumption) and Ventilatory Threshold in a dose dependent manner in the most recent trial on amateur runners. MAIN HUMAN NMN STUDY: https://jissn.biomedcentral. com/articles/10.1186/s12970- 021-00442-4 https://youtu.be/gi3vWDovF0Y - Video explaining this exact study and interpreting the results https://www.ncbi.nlm.nih.gov/ labs/pmc/articles/PMC7238909/ ElevATP ElevATP is a patented ingredient supported by several human trials in increasing performance and ATP production significantly. A randomized control trial demonstrated that a small dose of elevATP was able to increase systemic ATP by 20-70% 30-90min of supplementation in humans. The benefits of elevATP can be seen right after supplementation and does not require a 'loading phase'. elevATP can support increased work capacity and power output, which is especially useful in improving cardiovascular fitness. elevATP is FDA approved and is not banned in any sporting organizations. B, Argumedo R, Shu C, Huynh L, Pietrzkowski Z. Effect of the dietary supplement ElevATP on blood ATP level: An acute pilot clinical study. J Aging Res Clin Practice. 2013;2:178–84. Reyes-Izquierdo T, Shu C, Argumedo R, Nemzer B, Pietrzkowski Z. The effect of elevATPTM on whole blood ATP levels: a single dose, crossover clinical study. J Aging Res Clin Practice. 2014;3:56–60. Joy, J. M., Falcone, P. H., Vogel, R. M., Mosman, M. M., Kim, M. P., & Moon, J. R. (2015). Supplementation with a proprietary blend of ancient peat and apple extract may improve body composition without affecting hematology in resistance-trained men. Applied Physiology, Nutrition, and Metabolism, 40(11), 1171–1177. https://doi.org/10.1139/apnm- 2015-0241 Joy, J. M., Vogel, R. M., Moon, J. R., Falcone, P. H., Mosman, M. M., & Kim, M. P. (2016). Twelve weeks supplementation with an extended-release caffeine and ATP-enhancing supplement may improve body composition without affecting hematology in resistance-trained men. Journal of the International Society of Sports Nutrition, 13(1). doi:10.1186/s12970-016-0136-9 https://pubmed.ncbi.nlm.nih. gov/26489051 Sodium Nitrate Sodium Nitrate is popular within the cyclist community and has been shown to be a positive ergogenic aid in aerobic and anaerobic exercise in humans. Supplementing with nitrates is an effective way to increase nitric oxide production which leads to increased blood flow to the working tissues. Increasing tissue perfusion (blood flow) during exercise can help reduce the aerobic cost of exercise, leading to improved cardiovascular function and better endurance during exercise. Here are some interesting trials which support the benefits of nitrates in endurance sports: Nitrate supplementation's improvement of 10-km time-trial performance in trained cyclists - https://pubmed.ncbi.nlm.nih.gov/22248502/ Whole beetroot consumption acutely improves running performance- https://pubmed.ncbi.nlm.nih.gov/22709704/ Dietary nitrate supplementation improves team sport-specific intense intermittent exercise performance - https://pubmed.ncbi.nlm.nih.gov/23370859/ 125mcg 5-Methylfolate (Vitamin B9) + 500mcg Methylcobalamin (Vitamin B12) High doses of NMN (1000mg+) can cause increased homocysteine levels and decrease methyl donors in the body, which can lead to brain fog, cellular impairment in DNA replication and maintaining homeostasis in our organ systems. 5-methylfolate and methylcobalamin are both effective methyl donors which have been shown to replace the losses associated with NMN supplementation and attenuate the negative side effects of high dose/chronic use of all NAD+ precursors (including NMN). Cert. of Analysis CLICK HERE to view our product CoA - Thank You! Research https://www.nature.com/ articles/s41598-019-46120-z https://www.ncbi.nlm.nih.gov/ labs/pmc/articles/PMC3318212 / https://www.ncbi.nlm.nih.gov/ pmc/articles/PMC4425139/ Guarantee GUARANTEE RETURN POLICY GO2 Max is eligible for our "We Know You Will Love It" Guarantee which must meet the following conditions: The return must be requested within 60 days of receipt/delivery. The return is eligible for first-time buyers only. The return is eligible for single bottle purchases only. The return is only available for USA customers at this time. Empty bottles will not be considered for a refund. We suggest you try taking a 2-3 capsule serving if you do not feel the effects from just 1. The items must be returned with all original tags and packaging. Original shipping charges and Tax are non-refundable. Customers are responsible for the charges and tax of the return shipping, exchange shipping fee, and arrange return courier. HTLT Supplements will not accept any returned products without prior written communication via email and authorized confirmation. HTLT Supplements reserves the right to make the final decision on all cases. Online order cannot be exchanged or returned in physical store. After customer service department confirmed the exchange request, please return the return parcel within 7 days. Sale items cannot be returned or exchanged Upon tracking number being shared to our customer service team, we will send you a $20 gift card to htltsupps.com for the trouble. WAY TO INITIATE A RETURN Please send a request for a return authorization to our customer service email, [email protected] , within 60 days of receiving your order. You will receive a return authorization email and instructions once your return request is provisionally approved. Send your purchase back to us within 7 days after the return address is provided (Please note that customers are responsible for the return shipping fees). Once we have received your package, your refund will be processed within 30 business days. Online order cannot be exchange or return in physical store. Sale items cannot be returned or exchanged. Upon tracking number being shared to our customer service team, we will send you a $20 gift card to htltsupps.com for the trouble.
Turk Builder Max
Turk Builder MAX Is Here! NOW In Blister Packs & Made In Uzbekistan! That's right! After years of searching the globe for real, authentic Turkesterone, we discovered it in the mountains of Uzbekistan. We were able to create an extract that delivers real, authentic Turkesterone at 10mg per capsule and genuine 20HE at the same strength. Minor ecdysteroids are standardized to 4mg per capsules as well. All of this with the goal of giving YOU the #1 most effective Ecdysterone product available on the market. Note: For best shelf life, please store in a cool & dry place away from sunlight (ex: kitchen cupboard, medicine cabinet etc.) Directions Take one capsule to assess tolerance. Take 1-3 capsules 30 minutes before exercise on training days. Take 1-2 capsules in the morning on non training days. Do not exceed 3 capsules in one day. May affect sleep in some individuals when taken too close to bedtime, it is recommended not to take after 4pm. Supplement Facts Ingredients Uzbek Ajuga Turkestanica Extract (std . for Turkesterone & 20HE) Turkesterone and Ecdysteroids are structurally different from anabolic steroids and therefore work differently, ecdysteroids do not suppress natural sex hormone production and have not been shown to be toxic, they are found in very small percentages in foods like spinach and quinoa. Rhodiola Heterodonta Rhodiola Heterodonta is a perennial herb harvested from the high altitudes of the wilderness in Uzbekistan and Central Asia. It is a different species from the commonly known Rhodiola Rosea, grows in a different region and contains a unique spectrum of bioactive ingredients. According to Yousef et al . ( 2006) “R. heterodonta contained a newly detected phenylethanoid glycoside, heterodontoside, in addition to the known compounds tyrosol, viridoside, salidroside, and rhodiocyanoside A.” Our Rhodiola Heterodonta extract contains a high concentration of bioactive compounds (salidroside, flavonoids, organic acids, triterpenoids amongst others). Rhodiola Heterodonta may feel more stimulating than generic Rhodiola preparations on the market, we stand by the potency of this extract. Rhodiola Heterodonta is traditionally consumed as a stimulating agent during periods of exhaustive physical demand, Khojimatov et al (2023) states: “(it is) recommended as a herbal supplement in persons with an inclination to asthenia (especially in work requiring an increased mental load)”. This ingredient may potentiate the effects of Ajuga Turkestanica and compliment it well as they both are indicated for improving athletic performance and reducing fatigue. By expert opinion and dose experimentation, it is supported that Rhodiola Heterodonta is synergistic with Ajuga Turkestanica Extract and thus emerged as a popular combo with athletes in Uzbekistan. Capsorb (Sodium Caprate) Sodium caprate, a medium chain fatty acid, is one of the most effective pharmaceutical-grade absorption enhancers amongst its class. It increases tight junction permeability in the intestinal epithelium, thereby allowing for greater absorption of drugs/supplements with poor oral bioavailability.* Black Pepper Fruit Extract Black pepper is used to enhance bioavailability in supplements. There is no direct research that Black Pepper Extract directly helps the absorption of Ecdysterones, but it has been shown to increase absorption of vitamins and other nutrients. Piperine is a component of black pepper extract which is an established bioenhancer of many different nutraceuticals, it works by enhancing intestinal absorption of many poorly absorbed compounds and slowing the rate at which certain compounds are broken down by the liver; leading to increased serum concentrations and a greater AUC (area under the curve, related to pharmacokinetics).* Labs Labs backing label claims: Lab #1 For all batches, see Batch Report Repository Research Ismailov, S.I., Maksutova, N.N. (2015).Clinical study of the effectiveness and tolerability of the biologically active food supplement "Ecditon" produced by "BIOTON" LLC (Uzbekistan), in patients with type 2 diabetes mellitus. Endocrinology of the Ministry of Health of the Republic of Uzbekistan. https://drive.google.com/file/d/1tK8cu6L7q7W-xcF10Hzu9P0PctTMdHSv/view?usp=drive_link Sultanov, S.N., Mamatkasimov, A.M. (2014).Protocol for a clinical trial of a biologically active food supplement «Ecdirefin», in the Republican Specialized Scientific and Practical Medical Center for Obstetrics and Gynecology. https://drive.google.com/file/d/1eayRiKrsgDngatmUmdQiZ9WaODfR2Xdp/view?usp=drive_link Lawrence, M.M. (2012). Ajuga Turkestanica as a Countermeasure Against Sarcopenia and Dynapenia. Unpublished master’s thesis. Appalachian State University, Boone, NC. https://core.ac.uk/download/pdf/149240702.pdf Smetanin, B.Y. The Influence of Preparations of Plant Origin on Physical Work Capacity; The Russian Ministry of Public Health: Moscow, Russian, 1986. https://docs.google.com/document/d/1fZw3CM-U170X-TnDqNMIHALDIyD94jRs/edit?usp=sharing&ouid=115053821767806664957&rtpof=true&sd=true Simakin, S.Y.; Panyushkin, V.V.; Portugalov, S.N.; Kostina, L.V.; Martisorov, E.G. Combined application of preparation Ecdysten and product Bodrost during training in cyclic sports. Sports Sci. Bull. 1988, 2, 29–31. https://drive.google.com/file/d/1u7eNTnyhastHa-MbaLaY8EhOGOtQg6iR/view?usp=sharing Gadzhieva, R.M.; Portugalov, S.N.; Paniushkin, V.V.; Kondrat’eva, I.I. A comparative study of the anabolic action of Ecdysten, Leveton and Prime Plus, preparations of plant origin. Eksp Klin Farmakol. 1995, 58, 46–48. https://drive.google.com/file/d/1hDIG-nux1hybffQ9m807sKkYH97T0faV/view?usp=sharing Azizov, A.P.; Seifulla, R.D.; Ankudinova, I.A.; Kondrat’eva, I.I.; Borisova, I.G. Effect of the antioxidants elton and leveton on the physical work capacity of athletes. Eksp Klin Farmakol. 1998, 61, 60–62. https://drive.google.com/file/d/1FNpuHY12g9IMzhMcvwn1ZQnoouhoH4bg/view?usp=sharing Dustmukhamedova, D.K.H.; Kamilovz, A.T. The characteristic of energy metabolism disorders and its correction in children with celiac disease. Am. J. Med. Medic. Sci. 2020, 10, 780–783. https://drive.google.com/file/d/1R19U4LdPN8FL4sU7-fbEG25gmkjkYIZP/view?usp=sharing Seidlova-Wuttke, D.; Wuttke, W. In a placebo-controlled study β-ecdysone (ECD) prevented the development of the metabolic syndrome. Planta Med. 2012, 78, CL37. https://drive.google.com/file/d/1J-dDgrC4cYziLaHCrT3qS9zyrMq7xPm1/view?usp=sharing Emirova, L.R.; Rozhkova, E.A.; Paniushkin, V.V.; Mirzolan, R.S.; Seifulla, N.R. The effects of cytamines and their combinations with Ecdysten, Apilak, Vitamax and Essentiale on the work capacity of athletes. Eksp Klin Farmakol. 2004, 67, 66–68. https://drive.google.com/file/d/1Pgsq0rz5crfQM78ltvGJBzxsC8NY2edH/view?usp=sharing Semenov, V. Trial of the Leuzea-containing preparation «Rus-Olympic» under extreme conditions. In Leuzea and Your Health; Antoshechkin, A., Ed.; Ceptima Publishing Inc.: Clearwater, FL, USA, 2000. Safarova, D.D.; Tursunov, N.B. Aspects of sports medicine: The effect of Ekdisten. Sci. Sports Curr. Trends 2016, 12, 52–57. https://drive.google.com/file/d/1y6CcIQD7Gf1eaZ-ojGJ0tAuoYPEF4m1s/view?usp=sharing Isenmann, E.; Ambrosio, G.; Joseph, J.F.; Mazzarino, M.; de la Torre, X.; Zimmer, P.; Kazlauskas, R.; Goebel, C.; Botrè, F.; Diel, P.; et al. Ecdysteroids as non-conventional anabolic agent: Performance enhancement by ecdysterone supplementation in humans. Arch. Toxicol. 2019, 93, 1807–1816 http:/doi.org/10.1007/s00204-019-02490-x Lafont, R., Balducci, C., & Dinan, L. (2021). Ecdysteroids. Encyclopedia, 1(4), 1267–1302. https://doi.org/10.3390/encyclopedia1040096 Sarconeos—Biophytis—AdisInsight. (2022). https://adisinsight.springer.com/drugs/800045685 COVID-19: COVA clinical program. (n.d.). Biophytis. Retrieved July 2, 2022, from https://www.biophytis.com/en/pipeline/covid19-programme-clinique-cova/ Sarcopenia: SARA clinical program. (n.d.). Biophytis. Retrieved July 2, 2022, from https://www.biophytis.com/en/pipeline/plateforme-sarcob/ Sarconeos (BIO101). (n.d.). Biophytis. Retrieved July 2, 2022, from https://www.biophytis.com/en/our-science/sarcopenie/ Ecdysteroids as non-conventional anabolic agents: Pharmacodynamics, pharmacokinetics, and detection of ecdysterone. (2019). World Anti-Doping Agency. https://www.wada-ama.org/en/resources/funded-scientific-research/ecdysteroids-non-conventional-anabolic-agents-pharmacodynamics Syrov, V. (2006). Comparative experimental investigation of the anabolic activity of phytoecdysteroids and steranabols. Pharmaceutical Chemistry Journal. https://doi.org/10.1007/BF02524596 Lafont, R., Serova, M., Didry-Barca, B., Raynal, S., Guibout, L., Dinan, L., Veillet, S., Latil, M., Dioh, W., & Dilda, P. J. (2021). 20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor. Journal of Molecular Endocrinology, 68(2), 77–87. https://doi.org/10.1530/JME-21-0033 Figure 1) Davranov, Kakhramon & Jabborova, Dilfuza. (2020). Phytochemical, pharmacological and biological properties of Ajuga turkestanica (Rgl.) Brig (Lamiaceae). Annals of Phytomedicine: An International Journal. 9. 10.21276/p.2020.9.1.6. Twarog, C., Fattah, S., Heade, J., Maher, S., Fattal, E., & Brayden, D. J. (2019). Intestinal Permeation Enhancers for Oral Delivery of Macromolecules: A Comparison between Salcaprozate Sodium (SNAC) and Sodium Caprate (C10). Pharmaceutics, 11(2), 78. https://doi.org/10.3390/pharmaceutics11020078 Khojimatov, O.K., Khujanov, A.N., Bussmann, R.W. (2023). Rhodiola heterodonta (Hook.f. & Thomson) Boriss., Rhodiola pamiroalaica Boriss. - CRASSULACEAE . In: Khojimatov, O.K., Gafforov, Y., Bussmann, R.W. (eds) Ethnobiology of Uzbekistan. Ethnobiology. Springer, Cham. https://doi.org/10.1007/978-3-031-23031-8_64 Mr, Z., Sn, G., Rr, M., Aa, M., Nl, V., Ug, G., Sm, M., & Tf, A. (2024). Characterization of Rhodiola heterodonta (Crassulaceae): Phytocomposition, Antioxidant and Antihyperglycemic Activities. Preventive nutrition and food science, 29(2), 135–145. https://doi.org/10.3746/pnf.2024.29.2.135 Yousef, G. G., Grace, M. H., Cheng, D. M., Belolipov, I. V., Raskin, I., & Lila, M. A. (2006). Comparative phytochemical characterization of three Rhodiola species. Phytochemistry , 67 (21), 2380–2391. https://doi.org/10.1016/j.phytochem.2006.07.026 Guarantee GUARANTEE RETURN POLICY Turk Builder+ is eligible for our "We Know You Will Love It" Guarantee which must meet the following conditions: The return must be requested within 60 days of receipt/delivery. The return is eligible for first-time buyers only. The return is eligible for single bottle purchases only. The return is only available for USA customers at this time. Empty bottles will not be considered for a refund. We suggest you try taking a 2-3 capsule serving if you do not feel the effects from just 1. The items must be returned with all original tags and packaging. Original shipping charges and Tax are non-refundable. Customers are responsible for the charges and tax of the return shipping, exchange shipping fee, and arrange return courier. HTLT Supplements will not accept any returned products without prior written communication via email and authorized confirmation. HTLT Supplements reserves the right to make the final decision on all cases. Online order cannot be exchanged or returned in physical store. After customer service department confirmed the exchange request, please return the return parcel within 7 days. Sale items cannot be returned or exchanged Upon tracking number being shared to our customer service team, we will send you a $20 gift card to htltsupps.com for the trouble. WAY TO INITIATE A RETURN Please send a request for a return authorization to our customer service email, [email protected] , within 60 days of receiving your order. You will receive a return authorization email and instructions once your return request is provisionally approved. Send your purchase back to us within 7 days after the return address is provided (Please note that customers are responsible for the return shipping fees). Once we have received your package, your refund will be processed within 30 business days. Online order cannot be exchange or return in physical store. Sale items cannot be returned or exchanged. Upon tracking number being shared to our customer service team, we will send you a $20 gift card to htltsupps.com for the trouble.
CICO BAR Protein Bars
Description Sold as a 12-pack of protein bars. CICO is an acronym for one of our main philosophies on bettering personal health: Calories In, Calories Out. This Is Our Softest Formula Yet! We set out to create a bar that tasted AMAZING but did not compromise on calories. We wanted a bar that had taste and texture for e veryone to enjoy, but also left you feeling full. All units are sold as a box of 12 bars. ENTER: CICOBAR 230 Calories 20g Protein 33g Total Carbs 6g Fat Nutrition Facts Notes Important to note: in cold weather the bars get hard. Microwave for a few seconds to loosen the bar up. Allergen Warnings Contains ingredients from peanuts, milk, and soy. Made in a facility that also produces peanuts, milk, soy, wheat, tree nuts, and egg. Guarantee GUARANTEE RETURN POLICY CICO BAR is eligible for our "We Know You Will Love It" Guarantee which must meet the following conditions: The return must be requested within 60 days of receipt/delivery. The return is eligible for first-time buyers only. The return is eligible for single box purchases only. Empty boxes will not be considered for a refund. We suggest you try 2-3 bars. The items must be returned with all original tags and packaging. Original shipping charges and Tax are non-refundable. Customers are responsible for the charges and tax of the return shipping, exchange shipping fee, and arrange return courier. HTLT Supplements will not accept any returned products without prior written communication via email and authorized confirmation. HTLT Supplements reserves the right to make the final decision on all cases. Online order cannot be exchanged or returned in physical store. After customer service department confirmed the exchange request, please return the return parcel within 7 days. Sale items cannot be returned or exchanged Upon tracking number being shared to our customer service team, we will send you a $20 gift card to htltsupps.com for the trouble. WAY TO INITIATE A RETURN Please send a request for a return authorization to our customer service email, [email protected] , within 60 days of receiving your order. You will receive a return authorization email and instructions once your return request is provisionally approved. Send your purchase back to us within 7 days after the return address is provided (Please note that customers are responsible for the return shipping fees). Once we have received your package, your refund will be processed within 30 business days. Online order cannot be exchange or return in physical store. Sale items cannot be returned or exchanged. Upon tracking number being shared to our customer service team, we will send you a $20 gift card to htltsupps.com for the trouble.
Casein + Whey Protein
HTLT Supps ™ Drink, Bake, Sprinkle! HTLT Casein + Whey Protein was built to be the best tasting whey protein on the market. HTLT Whey protein can be made in a shake, baked into your favorite protein recipe, or even sprinkled onto your other foods. So good you could eat it with a spoon! You should be able to enjoy the whey protein you use... enter HTLT Casein + Whey Protein! HTLT Whey Protein is a Casein + whey concentrate blend. We chose this blend for better digestion, better flavor, and easier to use in your favorite protein recipes. *These statements have not been evaluated by the Food and Drug Administration (FDA). This product is not intended to diagnose, treat, cure, or prevent any disease.
G Test Platinum
Premium Test Booster! HTLT G-Test was created based on the 3 main ingredients found in our more simple 3-Test that have been made popular by some of the top scientists that have heavily researched the testosterone boosting category. G-Test is carefully researched, utilizing ingredients mostly backed by human studies. Learn more about our formula below! Supplement Facts Ingredients Tongkat Ali Extract Tongkat Ali is a well-known herb native to Southeast Asia, known for its therapeutic effects on sexual and hormonal health. Tongkat Ali contains a variety of bioactive compounds, including eurycomanones, glycosaponins, and polysaccharides, which have been shown to support increased sex drive, testosterone production, and improve sperm parameters in men [3] . A meta analysis done in 2021 by Lazarus et al evaluating 7 human trials found a positive net change in total testosterone levels with Tongkat Ali supplementation compared to placebo, in both hypo & eugonadal men. Lazarev, A., & Bezuglov, E. (2021). Testosterone Boosters Intake in Athletes: Current Evidence and Further Directions. Endocrines, 2(2), 109-120. https://doi.org/10.3390/endocrines2020011 Testosterone Boosters Intake in Athletes: Current Evidence and Further Directions Effect Study 1: A decrease of cortisol by 16%, and an increase of testosterone by +37% was noted in healthy adults. Study 2: a significant increase in testosterone (from 8.0 ± 1.6 ng/mL). Free testosterone also increased significantly (from 9.4 ± 3.3 to 12.6 ± 5.9 pg/mL). Trial Design Systematic Review: two RCTs on Tongkat Ali in healthy populations Trial Length Study 1: 4 weeks Study 2: 2 weeks Number of Subjects Study 1: 63 Study 2: 32 Population Study 1: Men and Women with moderate stress levels Study 2: Healthy men aged 24.4 ± 4.7 years Dosage Study 1: 200 mg/day of Physta™ Tongkat Ali Extract Study 2: 600mg/day Tongkat Ali extract Endocrines | Free Full-Text | Testosterone Boosters Intake in Athletes: Current Evidence and Further Directions. (n.d.). Retrieved March 18, 2022, from https://www.mdpi.com/2673-396X/2/2/11 How Does Tongkat Ali Work? Tongkat Ali extract works through a variety of mechanisms to support sexual and hormonal function. It is hypothesized that Tongkat’s active ingredients work as a 17α-hydroxylase activator, an aromatase inhibitor and demonstrates PDE5 inhibition to a notable degree [4,3] . By possible upregulation of 17α-hydroxylase, Tongkat Ali can increase the production of the sex hormones: pregnenolone, 17-OH-pregnenolone, DHEA, progesterone, 17-OH-progesterone, and 4-androstenedione [4] . This results in increased total testosterone & neurosteroid levels; on the other hand, by inhibiting the aromatase enzyme & enzymatic production of cortisol, Tongkat Ali can promote increases in total/free testosterone [1,2,3] . Ashwagandha Extract (Sensoril) Ashwagandha is an Ayurvedic herb historically used as an adaptogen. It can significantly increase testosterone levels in males and is thought to do so by downregulation of cortisol. The systematic review below provides an overview of the effects of this herb on male sex hormones and its safety profile. Examining the Effects of Herbs on Testosterone Concentrations in Men: A Systematic Review: Ashwagandha Effect Three of the 4 studies demonstrated positive effects of ashwagandha supplementation on testosterone concentrations in men. One study using KSM-66® was associated with a significant increase in testosterone concentrations (17.3% increase) after 90 d compared with the placebo group (3.8% increase) (P < 0.01). Another study showed that the ashwagandha group significantly increased testosterone concentrations (11.4%) compared with baseline values (P = 0.038) Trial Design Systematic Review; Three studies used a randomized, double-blind, placebo-controlled study design and 1 study used a randomized, double-blind, placebo-controlled, crossover study design Trial Length ~8 wks Number of Subjects 4 studies Population A total of 197 male participants were recruited for the studies, with ages ranging from 18 to 70 y and sample sizes ranging from 46 to 60 participants. Dosage Two studies used a patented ashwagandha root extract, KSM-66®, manufactured by Ixoreal (using a water-based extraction process, standardized to 5% withanolides) (35, 62), and the other 2 studies used another patented root and leaf extract, Shoden®, manufactured by Arjuna Natural (using a 70:30 ethanol:water extraction process, standardized to 35% withanolide glycosides). KSM study dosage: 600-675mg daily. Shoden® study dosage: 21 mg of withanolide glycosides daily Smith, S., Lopresti, A., Teo, S., & Fairchild, T. (2020). Examining the Effects of Herbs on Testosterone Concentrations in Men: A Systematic Review. Advances in Nutrition, 12. https://doi.org/10.1093/advances/nmaa134 D-Chiro Inositol D-Chiro Inositol is a cyclic sugar alcohol with biological activity as a second-messenger in the insulin signalling cascade, possessing anti-hyperglycemic and aromatase inhibiting properties, it has been shown to positively affect testosterone levels in males [6,7]. In a pilot trial on 10 healthy males, oral administration of D-chiro-inositol administration at 1g/day was associated with reduced serum levels of estrone (- 85.0%) and estradiol (- 14.4%), and increased serum levels of testosterone (+ 23.4%) and dehydroepiandrosterone (DHEA) (+ 13.8%) [7]. Additionally, epiandrosterone levels were higher (+39%) after treatment [7]. Fig. 1 Effect of D-chiro-inositol (DCI) treatment on total testosterone (T) levels. Total serum T levels from 10 male volunteers at baseline (t0) and after 30 days of treatment (t1) with 1 g DCI per day are presented as: a median values of total T (box plots). The boxes denote the interquartile range between the first and third quartiles (25th and 75th percentiles, respectively), the whiskers represent the minimum and maximum values, and the line inside the boxes represents the median value (p = 0.0020; Wilcoxon signed rank sum test);[7] Fig. 4 Effect of D-chiro-inositol (DCI) treatment on estrone (E1) levels. E1 levels from 10 male volunteers at baseline (t0) and after 30 days of treatment (t1) with 1 g DCI per day are presented as: a median values of E1 (box plots) (p = 0.0020; Wilcoxon signed rank sum test) [7] Fenugreek Extract Fenugreek is a spice which has an extensive use in food preparation and more recently sports supplementation, a recent meta analysis by Isenmann et al conducted a quantitative analysis on 6 human trials evaluating the effects of fenugreek preparations on sex hormones and athletic markers in healthy individuals; a statistically significant improvement was noted in total testosterone levels, free testosterone levels, and reductions of estradiol, stating that “chronic application of fenugreek has performance-enhancing and anabolic effects in male athletes” [19]. NAC (N-Acetyl-Cysteine) & Selenium In a randomized double blind placebo controlled trial published in The Journal of Urology by Safarinejad et al, it was found that administration of NAC (600mg/daily) and Selenium (200mcg/daily) in combination led to a significant increase in serum testosterone levels and improvement of sperm parameters in infertile men [20]. The exact mechanism is unknown, the authors state: “A prominent hypothesis is that decreased antioxidant function may be a contributing factor (of hypogonadism).”, indicating that the improvements in testicular function may be attributed to a reduction of oxidative stress affecting the leydig and sertoli cells of the testes [20]. Boron Boron is a trace mineral found in coffee, dairy, fruits and vegetables. Supplementation with boron has been shown to increase free testosterone levels in males, primarily through its effects on the metabolism of sex hormones and SHBG [8,9]. Comparative effects of daily and weekly boron supplementation on plasma steroid hormones and proinflammatory cytokines Effect Resulted in a statistically significant increase in the level of free testosterone, from 11.8 pg/mL to 15.2 pg/mL Trial Design Non-randomized controlled trial Trial Length 1 week Number of Subjects 8 Population Young males between the ages of 18-29 years old Dosage 10 mg daily Naghii, M. R., Mofid, M., Asgari, A. R., Hedayati, M., & Daneshpour, M.-S. (2011). Comparative effects of daily and weekly boron supplementation on plasma steroid hormones and proinflammatory cytokines. Journal of Trace Elements in Medicine and Biology: Organ of the Society for Minerals and Trace Elements (GMS), 25(1), 54–58. https://doi.org/10.1016/j.jtemb.2010.10.001 Shilajit Extract (std. 50% Fulvic Acid, PrimaVie) Shilajit is an ingredient derived from decomposed organic plant material and minerals, characterized by its richness in fulvic acid amongst other organic compounds [23]. It has a long history of use in South and East Asia for increasing vitality [23]. Trials evaluating Shilajit’s effects on males’ testosterone levels are promising, in which the greatest response was seen in hypogonadal men, seeing a +23.5% improvement in total testosterone levels [21]. In another human trial, Shilajit administration was also shown to significantly increase total testosterone, free testosterone and DHEA-S levels in healthy volunteers compared to placebo [22]. Wild Yam Extract (std. for Diosgenin) Wild yam extract contains a significant amount of Diosgenin, a steroidal saponin which has been studied in humans as being a beneficial natural compound for improving athletic performance and increasing sex hormone levels in healthy males [18]. In a randomized placebo controlled trial, Diosgenin supplementation combined with resistance training increased serum DHEA, serum DHT, serum free testosterone, increased 1-rep max on the deadlift and increased arm fat-free mass compared to placebo [18]. Horii, N., Hasegawa, N., Fujie, S., Iemitsu, K., Uchida, M., Hamaoka, T., & Iemitsu, M. (2020). Effects of Dioscorea esculenta intake with resistance training on muscle hypertrophy and strength in sprint athletes. Journal of Clinical Biochemistry and Nutrition , 67(3), 338–343. https://doi.org/10.3164/jcbn.19-124 Zinc Citrate Zinc deficiency can be a cause of low testosterone levels in men, zinc supplementation has been shown to reliably increase testosterone production in zinc-deficient men and is a first line intervention when trying to naturally increase testosterone levels in the context of nutrient deficiency [10,11]. Zinc status and serum testosterone levels of healthy adults Effect Zinc supplementation of marginally zinc-deficient normal elderly men for six months resulted in an increase in serum testosterone from 8.3 +/- 6.3 to 16.0 +/- 4.4 nmol/L (p = 0.02). Trial Design Comparative Study Trial Length 6 Months Number of Subjects 40 men Population 20 to 80 y of age Dosage 459 mumol (30mg) of elemental zinc (administered as zinc gluconate) per day As, P., Cs, M., Fw, B., Jw, H., & Gj, B. (1996). Zinc status and serum testosterone levels of healthy adults. Nutrition (Burbank, Los Angeles County, Calif.), 12(5). https://doi.org/10.1016/s0899-9007(96)80058-x P5P (Pyridoxal phosphate, active form of vitamin B6) The active form of vitamin B6, P5P, has been shown to improve outcomes in the context of hyperprolactinemia, a possible endocrine cause of hormonal suppression (excess prolactin secretion results in secondary hypogonadism) [12,13,14]. Although the trials evaluating Vitamin B6/P5P’s effects on hyperprolactinemia used a very high dosage (300mg q12h PO), we felt a lower dose was warranted as the degree of hyperprolactinemia experienced by the participants in this trial was pharmacologically induced (secondary to antipsychotic use), above and excess of what might exist in individuals who do not have prolactinomas or concurrent use of antipsychotics. The dosage we used was less than the studied dosage with intent to target a favorable safety profile (some individuals can experience peripheral neuropathy with very high doses of vitamin B6). Vitamian B6 (P5P): Safety and Efficacy of High-Dose Vitamin B6 as an Adjunctive Treatment for Antipsychotic-Induced Hyperprolactinemia in Male Patients With Treatment-Resistant Schizophrenia Effect Vitamin B6 supplementation led to an average of a 68.1% reduction in serum prolactin levels. Trial Design Randomized double-blinded controlled study Trial Length 16 weeks Number of Subjects 200 Population Patients with Treatment-Resistant-Schizophrenia, adult males between 20 and 40 years of age. Dosage 300 mg/12 h Zhuo, C., Xu, Y., Wang, H., Fang, T., Chen, J., Zhou, C., Li, Q., Liu, J., Xu, S., Yao, C., Yang, W., Yang, A., Li, B., Chen, Y., Tian, H., & Lin, C. (2021). Safety and Efficacy of High-Dose Vitamin B6 as an Adjunctive Treatment for Antipsychotic-Induced Hyperprolactinemia in Male Patients With Treatment-Resistant Schizophrenia. Frontiers in Psychiatry, 12. Vitamin D3 Vitamin D3 deficiency is a common global health issue which exists primarily in higher latitudes where individuals may not get adequate sunlight exposure throughout the year, some forms of secondary hypogonadism have a strong association with vitamin D deficiency [16]. Correcting deficient vitamin D levels is a safe and effective way to promote optimal health outcomes, especially in the context of deficiency. G Test+ implements a dosage in line with the RDA for vitamin D3 as a conservative approach, to reduce the risk of hypervitaminosis D for those who already consume a daily vitamin D supplement or plan to take this perpetually (fat-soluble vitamins can accumulate in tissues if taken in excess for long durations and can lead to toxicity if levels become too high). Effect of vitamin D supplementation on testosterone levels in men Effect Vitamin D supplementation led to significant increases in total testosterone levels (from 10.7±3.9 nmol/l to 13.4±4.7 nmol/l; p<0.001), bioactive testosterone (from 5.21±1.87 nmol/l to 6.25±2.01 nmol/l; p=0.001), and free testosterone levels (from 0.222±0.080 nmol/l to 0.267±0.087 nmol/l; p=0.001). The placebo group did not experience any significant changes in testosterone measures. Trial Design Randomized Control Trial Trial Length 12 months Number of Subjects 54 Population Men (age range not specified, greater than 20+ years old) Dosage 3,332 IU/day Pilz, S., Frisch, S., Koertke, H., Kuhn, J., Dreier, J., Obermayer-Pietsch, B., Wehr, E., & Zittermann, A. (2011). Effect of Vitamin D Supplementation on Testosterone Levels in Men. Hormone and Metabolic Research, 43(3), 223–225. https://doi.org/10.1055/s-0030-1269854 Research Chan, K. Q., Stewart, C., Chester, N., Hamzah, S. H., & Yusof, A. (2021). The effect of Eurycoma Longifolia on the regulation of reproductive hormones in young males. Andrologia , 53 (4). https://doi.org/10.1111/and.14001 Leisegang, K., Finelli, R., Sikka, S. C., & Selvam, M. K. P. (2022). Eurycoma longifolia (Jack) Improves Serum Total Testosterone in Men: A Systematic Review and Meta-Analysis of Clinical Trials. Medicina , 58 (8). https://doi.org/10.3390/medicina58081047 Rehman, S. U., Choe, K., & Yoo, H. H. (2016). Review on a Traditional Herbal Medicine, Eurycoma longifolia Jack (Tongkat Ali): Its Traditional Uses, Chemistry, Evidence-Based Pharmacology and Toxicology. Molecules , 21 (3), Article 3. https://doi.org/10.3390/molecules21030331 Eng, A. (2007). Correcting systemic androgen levels using Eurycoma longifolia (United States Patent No. US20070009621A1). https://patents.google.com/patent/US20070009621A1/en Chinnappan, S. M., George, A., Pandey, P., Narke, G., & Choudhary, Y. K. (2021). Effect of Eurycoma longifolia standardised aqueous root extract-Physta® on testosterone levels and quality of life in ageing male subjects: a randomised, double-blind, placebo-controlled multicentre study. Food & nutrition research, 65, 10.29219/fnr.v65.5647. https://doi.org/10.29219/fnr.v65.5647 Montt-Guevara, M. M., Finiguerra, M., Marzi, I., Fidecicchi, T., Ferrari, A., Genazzani, A. D., & Simoncini, T. (2021). D-Chiro-Inositol regulates insulin signaling in human adipocytes. Frontiers in Endocrinology, 12, Article 660815. https://doi.org/10.3389/fendo.2021.660815 Monastra, G., Vazquez-Levin, M., Bezerra Espinola, M.S. et al. D-chiro-inositol, an aromatase down-modulator, increases androgens and reduces estrogens in male volunteers: a pilot study. Basic Clin. Androl. 31, 13 (2021). https://doi.org/10.1186/s12610-021-00131-x Naghii, M. R., Mofid, M., Asgari, A. R., Hedayati, M., & Daneshpour, M.-S. (2011). Comparative effects of daily and weekly boron supplementation on plasma steroid hormones and proinflammatory cytokines. Journal of Trace Elements in Medicine and Biology: Organ of the Society for Minerals and Trace Elements (GMS), 25(1), 54–58. https://doi.org/10.1016/j.jtemb.2010.10.001 Pizzorno L. Nothing Boring About Boron. Integr Med (Encinitas). 2015 Aug;14(4):35-48. PMID: 26770156; PMCID: PMC4712861. Khan, S. S., Carter, R. E., & Greenland, P. (2023). Association of anemia with cardiovascular disease: A cohort study. The American Journal of Medicine. Advance online publication. https://doi.org/10.1016/j.amjmed.2023.01.045 As, P., Cs, M., Fw, B., Jw, H., & Gj, B. (1996). Zinc status and serum testosterone levels of healthy adults. Nutrition (Burbank, Los Angeles County, Calif.), 12(5). https://doi.org/10.1016/s0899-9007(96)80058-x Zhuo, C., Xu, Y., Wang, H., Fang, T., Chen, J., Zhou, C., Li, Q., Liu, J., Xu, S., Yao, C., Yang, W., Yang, A., Li, B., Chen, Y., Tian, H., & Lin, C. (2021). Safety and Efficacy of High-Dose Vitamin B6 as an Adjunctive Treatment for Antipsychotic-Induced Hyperprolactinemia in Male Patients With Treatment-Resistant Schizophrenia. Frontiers in Psychiatry, 12. Witwit, Suha. (2018). The Role of Vitamin B6 in Reducing Serum Prolactin in Comparison to Cabergoline. International Journal of Pharmaceutical Quality Assurance. 10. 10.25258/ijpqa.10.1.17. Araújo, A. N., Wessling, A., & Bugalho, M. J. (2018). Male hypogonadism – when two endocrine causes merge in the same patient. Endocrine Abstracts, 56, P810. https://doi.org/10.1530/endoabs.56.P810 Janz, T., & Pearson, C. (2013). Vitamin D blood levels of Canadians. Health at a Glance. Statistics Canada Catalogue no. 82-624-X. https://www150.statcan.gc.ca/n1/pub/82-624-x/2013001/article/11727-eng.htm Lee, D. M., Tajar, A., Pye, S. R., Boonen, S., Vanderschueren, D., Bouillon, R., O'Neill, T. W., Bartfai, G., Casanueva, F. F., Finn, J. D., Forti, G., Giwercman, A., Han, T. S., Huhtaniemi, I. T., Kula, K., Lean, M. E., Pendleton, N., Punab, M., Wu, F. C., & EMAS study group (2012). Association of hypogonadism with vitamin D status: the European Male Ageing Study. European journal of endocrinology, 166(1), 77–85. https://doi.org/10.1530/EJE-11-0743 Pilz, S., Frisch, S., Koertke, H., Kuhn, J., Dreier, J., Obermayer-Pietsch, B., Wehr, E., & Zittermann, A. (2011). Effect of Vitamin D Supplementation on Testosterone Levels in Men. Hormone and Metabolic Research, 43(3), 223–225. https://doi.org/10.1055/s-0030-1269854 Horii, N., Hasegawa, N., Fujie, S., Iemitsu, K., Uchida, M., Hamaoka, T., & Iemitsu, M. (2020). Effects of Dioscorea esculenta intake with resistance training on muscle hypertrophy and strength in sprint athletes. Journal of Clinical Biochemistry and Nutrition, 67(3), 338–343. https://doi.org/10.3164/jcbn.19-124 Isenmann, E., Alisauskas, P., Flenker, U., Schalla, J., & Diel, P. (2023). The Anabolic Effect of Fenugreek: A Systematic Review with Meta-analysis. International journal of sports medicine, 44(10), 692–703. https://doi.org/10.1055/a-2048-5925 Safarinejad, M. R., & Safarinejad, S. (2009). Efficacy of selenium and/or N-acetyl-cysteine for improving semen parameters in infertile men: a double-blind, placebo controlled, randomized study. The Journal of urology, 181(2), 741–751. https://doi.org/10.1016/j.juro.2008.10.015 Biswas, T. K., Pandit, S., Mondal, S., Biswas, S. K., Jana, U., Ghosh, T., Tripathi, P. C., Debnath, P. K., Auddy, R. G., & Auddy, B. (2010). Clinical evaluation of spermatogenic activity of processed Shilajit in oligospermia. Andrologia, 42(1), 48–56. https://doi.org/10.1111/j.1439-0272.2009.00956.x Pandit, S., Biswas, S., Jana, U., De, R. K., Mukhopadhyay, S. C., & Biswas, T. K. (2016). Clinical evaluation of purified Shilajit on testosterone levels in healthy volunteers. Andrologia, 48(5), 570–575. https://doi.org/10.1111/and.12482 Carrasco-Gallardo, C., Guzmán, L., & Maccioni, R. B. (2012). Shilajit: a natural phytocomplex with potential procognitive activity. International journal of Alzheimer's disease, 2012, 674142. https://doi.org/10.1155/2012/674142
Creatine Monohydrate
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